Compound Light Microscope Objective Lens Magnification

Typically a lower magnification objective lens will have a larger field of view and a higher magnification objective lens will have a smaller field of view.

Compound light microscope objective lens magnification.

The eyepiece and an objective lens. A compound microscope has multiple lenses. The objective lens typically 4x 10x 40x or 100x is compounded multiplied by the eyepiece lens typically 10x to obtain a high magnification of 40x 100x 400x and 1000x. The standard objective lenses magnify 4x 10x and 40x.

Microscopes enlarge or magnify the image of an object. Higher magnification is achieved by using two lenses rather than just a single magnifying lens. An important point to understand when working with compound microscopes and their objective lenses is that the field of view changes as the magnification changes. The common ocular magnifies ten times marked as 10x.

Therefore a 10x eyepiece used with a 40x objective lens will produce a magnification of 400x. A compound microscope will normally have around three to five objective lenses each with a magnification of 4x to 100x. If you view a specimen at 40x total magnification with a 10x ocular lens then you are viewing the specimen with what objective. These lenses are located on the rotating nosepiece and are the most crucial in magnifying the specimen in order to see it bigger better and in more detail.

To calculate total magnification find the magnification of both the eyepiece and the objective lenses. 4x a compound microscope uses two lenses at once to magnify the image of a specimen. Calculate the magnification by multiplying the eyepiece magnification usually 10x by the objective magnification usually 4x 10x or 40x. Scanning objective lens 4x combined with the eyepiece lens this lens will provide the lowest magnification power.

If the microscope has a fourth objective lens the magnification will most likely be 100x. The majority of compound microscopes come with interchangeable objective lenses which have different magnification powers. Calculating the magnification of light microscopes the compound microscope uses two lenses to magnify the specimen. Light microscopes combine the magnification of the eyepiece and an objective lens.

Magnification in order to ascertain the total magnification when viewing an image with a compound light microscope take the power of the objective lens which is at 4x 10x or 40x and multiply it by the power of the eyepiece which is typically 10x. The total magnification of 40 means that the object appears forty times larger than the actual object. This commonly includes 4x 10x 40x and 100x objective lenses. The maximum useful magnification of a light microscope is 1 500x.